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Updated: Jul 4, 2026

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
Published on: April 18, 2025
Evaluation of the variables characterized by significant discriminating power in the application of SIMCA
Emilio Marengo1, Elisa Robotti, Marco Bobba
1Department of Environmental and Life Sciences, University of Eastern Piedmont, Via Bellini 25/G, 15100 Alessandria, Italy. marengoe@tin.it
Abstract:
SIMCA classification can be applied to 2D-PAGE maps to identify changes occurring in cellular protein contents as a consequence of illnesses or therapies. These data sets are complex to treat due to the large number of proteins detected. A method for identifying relevant proteins from SIMCA discriminating powers is proposed, based on the Box-Cox transformation coupled to probability papers. The method successfully allowed the identification of the relevant spots from 2D maps.
