Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring tumor dynamics and responses of prostate cancer to IL-27 based treatment combinations through biodynamic imaging and RNA sequencing analyses.

Scientific reports·2025
Same author

Fresnel biprism common-path low-coherence digital holography for dynamic light scattering spectroscopy of biological materials.

Biomedical optics express·2025
Same author

Coherent light scattering from cellular dynamics in living tissues.

Reports on progress in physics. Physical Society (Great Britain)·2024
Same author

Biodynamic prediction of neoadjuvant chemotherapy response: Results from a prospective multicenter study of predictive accuracy among muscle-invasive bladder cancer patients.

Urologic oncology·2022
Same author

Cancer Holography for Personalized Medicine.

Optics and photonics news·2022
Same author

Biodynamic digital holographic speckle microscopy for oocyte and embryo metabolic evaluation.

Applied optics·2021
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Molecular interferometric imaging.

Ming Zhao1, Xuefeng Wang, David D Nolte

  • 1Department of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, IN 47907, USA.

Optics Express
|June 12, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a novel surface metrology technique using common-path interferometry for high-resolution surface analysis. The biosensor achieves 15 picometer resolution, enabling sensitive detection in immunoassays.

More Related Videos

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
09:45

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Related Experiment Videos

Last Updated: Jul 4, 2026

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
06:43

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Published on: May 3, 2022

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)
11:04

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor (IRIS)

Published on: May 3, 2011

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
09:45

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Area of Science:

  • Surface Metrology
  • Biosensing Technology

Background:

  • Accurate surface characterization is crucial for advanced material science and diagnostics.
  • Existing metrology techniques often lack the required resolution or sensitivity for nanoscale applications.

Purpose of the Study:

  • To develop a high-resolution surface metrology system using common-path in-line shearing interferometry.
  • To demonstrate the application of this system for sensitive biosensing, specifically in immunoassays.

Main Methods:

  • Utilized common-path in-line shearing interferometry with pixel-array imaging.
  • Employed an eighth-wave thermal oxide on silicon for phase quadrature reference wave generation.
  • Applied the system to antibody microarray formats for reverse-phase immunoassays.

Main Results:

  • Achieved 15 picometer (pm) surface height resolution.
  • Demonstrated a scaling surface mass sensitivity of 7 fg/mm at 40x magnification.
  • Attained a limit of detection of 10 ng/ml for immunoassays with 1-hour incubation.
  • Exhibited a mass sensitivity of 2 pg/mm² for real-time binding measurements.

Conclusions:

  • The developed interferometric surface metrology offers exceptional resolution and sensitivity.
  • The biosensor platform is suitable for real-time, high-sensitivity detection in immunoassay applications.
  • Further improvements in biosensing sensitivity are limited by biological and chemical variability.