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A PDMS-based disposable microfluidic sensor for CD4+ lymphocyte counting.

Sara Thorslund¹, Rolf Larsson², Jonas Bergquist³

¹Department of Engineering Sciences, Ångström Laboratory, Uppsala University, Box 534, 751 21, Uppsala, Sweden. sara.thorslund@angstrom.uu.se.

Biomedical Microdevices

|June 12, 2008

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A new CD4+ lymphocyte sensor using capillary action was developed. Dilution media significantly impacts cell counts, highlighting the need for controlled testing in clinical settings.

Area of Science:

Biomedical Engineering
Immunology
Microfluidics

Background:

Accurate CD4+ lymphocyte counts are crucial for managing immune deficiencies.
Existing methods like flow cytometry can be complex and require specialized equipment.
Development of portable, user-friendly diagnostic tools is needed.

Purpose of the Study:

To develop and evaluate a novel microfluidic sensor for CD4+ lymphocyte quantification.
To assess the sensor's performance against standard flow cytometry.
To investigate the impact of sample preparation and dilution media on sensor accuracy.

Main Methods:

Fabrication of a PDMS-based micropillar structured sensor.
Surface modification for biocompatibility and CD4+ cell capture.

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Capillary-driven filling and rinsing without external pumps.

Comparison of sensor results with flow cytometry using whole blood and diluted samples.

Main Results:

The developed sensor demonstrated acceptable agreement (79%) with flow cytometry for whole blood.
Diluting blood in PBS buffer led to increased cell counts due to shear-induced migration.
The choice of diluent (plasma, PBS with glycerol/albumin) significantly altered cell behavior.
Importance of controlling dilution media for capillary-driven microfluidic devices was confirmed.

Conclusions:

The capillary-driven microfluidic sensor shows promise for CD4+ lymphocyte counting.
Careful consideration of dilution media is essential to mitigate cell migration artifacts.
Further validation with clinically relevant low CD4+ counts is required.