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Isolation and partial characterization of mitogenic factors from cementum
H Nakae1, A S Narayanan, E Raines
1Department of Pathology, University of Washington, Seattle 98195.
Biochemistry
|July 23, 1991
Summary
Researchers identified a novel cementum growth factor (CGF) in bovine cementum that stimulates fibroblast proliferation. This CGF, distinct from platelet-derived growth factor, shows potential for applications in regenerative dentistry.
Area of Science:
- Biochemistry
- Cell Biology
- Dental Research
Background:
- Cementum is a mineralized dental tissue crucial for tooth-to-connective tissue attachment.
- It contains bioactive substances influencing adjacent soft tissue cells, like fibroblasts.
- Previous characterization of cementum proteins with mitogenic activity was limited.
Purpose of the Study:
- To partially characterize cementum proteins exhibiting mitogenic activity toward fibroblasts.
- To identify and isolate the specific factors responsible for this proliferative effect.
- To compare the properties of cementum mitogens with those found in bone.
Main Methods:
- Extraction of mitogenic factors from bovine cementum using acetic acid.
- Fractionation using Heparin-Sepharose chromatography and High-Performance Liquid Chromatography (HPLC).
- Identification of proteins via SDS-polyacrylamide gel electrophoresis and Western blotting with anti-bFGF antibody.
Main Results:
- Two mitogenic fractions were isolated, one identified as basic fibroblast growth factor (bFGF).
- A major mitogen, designated CGF (Mr 22,000), was purified and showed potentiated activity with serum or EGF.
- CGF was heat resistant but sensitive to reduction, and lacked detectable platelet-derived growth factor.
Conclusions:
- Bovine cementum contains distinct mitogenic factors, including bFGF and a novel CGF.
- CGF exhibits unique properties and is distinct from platelet-derived growth factor.
- These findings suggest CGF's potential role in regulating fibroblast behavior and in tissue regeneration.