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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.Ribosome Structure and AssemblyRibosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within the...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...
Ribosomes01:27

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome production. Within...

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Updated: Jul 4, 2026

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
10:27

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Correlating ribosome function with high-resolution structures.

Anat Bashan1, Ada Yonath

  • 1Department of Structural Biology, Weizmann Institute, Rehovot, 76100, Israel.

Trends in Microbiology
|June 13, 2008
PubMed
Summary
This summary is machine-generated.

Recent advances in ribosome research reveal its intricate architecture and functions. Structural insights explain precise decoding, peptide bond formation, and antibiotic mechanisms.

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Last Updated: Jul 4, 2026

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution
10:27

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Published on: July 8, 2019

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
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Area of Science:

  • Molecular Biology
  • Structural Biology
  • Biochemistry

Background:

  • Ribosome research has seen significant advancements.
  • Crystal structures illuminate the translation machinery's functional properties.

Purpose of the Study:

  • To elucidate the ribosome's architecture and functional capabilities.
  • To detail the mechanisms of tRNA translocation and nascent-chain processing.
  • To understand antibiotic interactions with the ribosome.

Main Methods:

  • X-ray crystallography
  • Cryo-electron microscopy (Cryo-EM)
  • Biochemical assays

Main Results:

  • Detailed crystal structures of the ribosome.
  • Elucidation of tRNA translocation mechanisms (sideways shift and rotatory motion).
  • Insights into the nascent-chain exit tunnel dynamics and trigger factor function.
  • Understanding of antibiotic action, selectivity, resistance, and synergism.

Conclusions:

  • Ribosome structure is key to its precise decoding, peptide-bond formation, and polymerase activity.
  • Structural data provides a framework for understanding ribosome-mediated processes.
  • Insights into antibiotic targeting of ribosomes are enhanced by structural information.