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Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
A study of cellobiose fermentation by a Dekkera strain
B Blondin1, R Ratomahenina, A Arnaud
1Chaire de Génétique et Microbiologie, ENSA-INRA Place Viala, 34060 Montpellier Cedex, France.
Biotechnology and Bioengineering
|September 1, 1982
Summary
The Dekkera intermedia yeast strain efficiently ferments cellobiose into ethanol, achieving a high yield. However, concentrated cellobiose solutions hindered the fermentation process.
Area of Science:
- Microbiology
- Biotechnology
- Fermentation Science
Background:
- Cellobiose is a disaccharide derived from cellulose hydrolysis.
- Efficient fermentation of cellobiose is crucial for biofuel production.
- Yeast strains capable of cellobiose fermentation are of significant industrial interest.
Purpose of the Study:
- To investigate the cellobiose fermentation capabilities of the Dekkera intermedia strain.
- To determine the optimal conditions for ethanol production from cellobiose.
Main Methods:
- Culturing of Dekkera intermedia.
- Fermentation experiments using varying concentrations of cellobiose.
- Measurement of ethanol concentration and yield.
Main Results:
- Dekkera intermedia successfully fermented cellobiose to produce ethanol.
- An ethanol concentration of 75 g/L was achieved from 180 g/L cellobiose, representing 80% of the theoretical yield.
- Fermentation efficiency decreased with higher cellobiose concentrations.
Conclusions:
- Dekkera intermedia demonstrates a notable capacity for cellobiose fermentation.
- The strain shows potential for bioethanol production, but substrate concentration requires optimization.
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