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Electronic Tongue Generating Continuous Recognition Patterns for Protein Analysis
Published on: September 16, 2014
Resonance light-scattering enhancement effect of the protein-Y3+-TTA-SLS system and its analytical application
Changying Guo1, Xia Wu, Wei Xu
1Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, People's Republic of China.
Abstract:
In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris-HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y(3+)-2-thenoyltrifluoroacetone (TTA)-sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 x 10(-9)-1.0 x 10(-5) g/mL for BSA, 1.0 x 10(-8)-1.0 x 10(-5) g/mL for HSA and 1.0 x 10(-8)-1.0 x 10(-6 )g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied.
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