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The dual roles of Ganoderma antioxidants on urothelial cell DNA under carcinogenic attack

J W M Yuen1, M D I Gohel

  • 1Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China. htjohn@inet.polyu.edu.hk

Journal of Ethnopharmacology
|June 14, 2008
PubMed
Abstract

Insights

Ganoderma lucidum extracts show dual effects on oxidative DNA damage. The water-soluble extract protected against damage, while the water-insoluble extract induced it, suggesting complex roles in bladder cancer prevention.

Area of Science:

  • Pharmacology
  • Biochemistry
  • Traditional Chinese Medicine

Background:

  • Ganoderma lucidum (Lingzhi) exhibits antioxidant and anti-cancer properties.
  • Oxidative stress and DNA damage are implicated in bladder cancer development.
  • Understanding the role of Lingzhi antioxidants in bladder chemoprevention is crucial.

Purpose of the Study:

  • To investigate the effects of Lingzhi antioxidants on oxidative DNA damage and oxidative stress.
  • To elucidate the dual roles of Ganoderma lucidum components in bladder chemoprevention.
  • To explore the relationship between antioxidant-oxidant balance and traditional Chinese medicine principles.

Main Methods:

  • Tested antioxidant-enhanced extracts for ferric reducing antioxidant power (FRAP) and DPPH inhibition.
  • Assessed hydrogen peroxide (H(2)O(2)) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations.
  • Utilized an in vitro pre-cancerous human uroepithelial cell (HUC-PC) tumorigenic model.

Main Results:

  • Water-soluble extract (GLw) showed higher antioxidant capacity than water-insoluble extract (GLe).
  • GLw reduced 8-OHdG levels in HUC-PC cells challenged with 4-aminobiphenyl (ABP).
  • GLe induced H(2)O(2) and 8-OHdG formation in a dose-dependent manner.

Conclusions:

  • Ganoderma lucidum components exhibit dual roles in oxidative DNA damage.
  • Oxidative DNA damage may underlie Lingzhi-induced apoptosis in bladder chemoprevention.
  • The findings align with traditional Chinese medicine's Yin-Yang balance theory.

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