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An enzyme-chromogenic surface plasmon resonance biosensor probe for hydrogen peroxide determination using a modified
Hideaki Nakamura1, Yotaro Mogi, Takuo Akimoto
1School of Bioscience and Biotechnology, Tokyo University of Technology, Hachioji, Tokyo, Japan. nakamura@bs.teu.ac.jp
Biosensors & Bioelectronics
|June 14, 2008
Summary
A novel absorption-based surface plasmon resonance (SPR(Abs)) biosensor enables simple hydrogen peroxide detection using a modified Trinder's reagent. This method offers a sensitive and reproducible platform for biosensing applications.
Area of Science:
- Biosensing
- Analytical Chemistry
- Spectroscopy
Background:
- Surface Plasmon Resonance (SPR) is a powerful technique for detecting molecular interactions.
- Hydrogen peroxide (H2O2) is a key analyte in various biological and chemical processes.
- Existing methods for H2O2 detection can be complex or lack sensitivity.
Purpose of the Study:
- To develop a simple and reproducible absorption-based SPR (SPR(Abs)) biosensor for hydrogen peroxide detection.
- To utilize a modified Trinder's reagent for sensitive colorimetric H2O2 determination.
- To assess the applicability of the SPR(Abs) biosensor for other analyte detections.
Main Methods:
- Development of an SPR(Abs) biosensor probe.
- Utilized a modified Trinder's reagent involving N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethylaniline sodium salt monohydrate (MAOS) and 4-aminoantipyrine (4-AA).
- Direct application of a 5mM hydrogen peroxide solution to the SPR sensor probe for measurement.
Main Results:
- The SPR(Abs) biosensor achieved a high absorbance value (46.7 cm(-1)) for 5mM hydrogen peroxide.
- A linear calibration curve was obtained for hydrogen peroxide concentrations between 1.0 and 50mM (r=0.991).
- A detection limit of 0.5mM for hydrogen peroxide was achieved with high reproducibility (RSD=0.152%).
- Demonstrated applicability for glucose detection using glucose oxidase, unaffected by refractive index changes.
Conclusions:
- The developed SPR(Abs) biosensor probe provides a simple, reproducible, and sensitive method for hydrogen peroxide quantification.
- The modified Trinder's reagent facilitates efficient color development for SPR(Abs) detection.
- The biosensor platform shows potential for detecting various analytes beyond hydrogen peroxide.
