Thin layer chromatography analysis of antioxidant constituents of lichens from Antarctica

Antioxidant agents against reactive oxygen species can be used for several cosmetic and medicinal applications. Methanol-water (90:10 v/v) extracts of five polar lichen species--namely Stereocaulon alpinum Laurer (Stereocaulaceae); Ramalina terebrata Hook and Taylor (Ramalinaceae); Caloplaca sp. (Teloschistaceae); Lecanora sp. (Lecanoraceae); and Caloplaca regalis (Vain.)Zahlbr (Teloschistaceae) from King George Island (Antarctica)--were analyzed using thin layer chromatography (TLC) followed by a DPPH (2,2-diphenyl-1-picrylhydrazyl) spray technique. The experimental data showed that 33-50% of the major constituents of the test extracts were active antioxidants. Stereocaulon alpinum and R. terebrata showed a higher number (50%) of antioxidant constituents, although their activities were comparatively weak. The strength of antioxidant activity in terms of discoloration of DPPH was shown to be stronger by the constituents of S. alpinum, C. regalis and C. sp. In addition, phenolic content in these Antarctic lichen extracts was in the range of 17-47 mg/g, supporting the antioxidant data of TLC analysis. Thus, these results suggest that Antarctic lichen contains a variety of strong antioxidant constituents. Therefore, further study of the laboratory culture of lichen is warranted to investigate possible commercial production, followed by isolation and characterization of the active antioxidant agents, which can be used against various oxidative stress-related diseases.