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Updated: Jul 4, 2026
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
Published on: January 31, 2014
DNA immobilized onto an acyl-azide derivative of collagen membranes for use as immunoadsorbent
R El Habib1, P R Coulet, K Sanhadji
1Hôpital Edouard Herriot Pavillon P, Unité 80 Inserm, Place d'Arsonval, 69374 Lyon Cedex 08, France.
Abstract:
Single- and double-stranded DNA were immobilized on films of highly polymerized collagen by a covalent process. The coupling was efficient at an acidic pH with an optimum at pH 5, while preventing the collagen film from any damage. In addition, no leakage occurred, so it was possible to use this DNA-coated collagen film as an immunoadsorbent. Therefore the findings reported here suggest that the acyl-azide procedure is also suitable for DNA binding on a proteinic support. Promising results in specific clearance of DNA antibodies were obtained in vitro.
