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Updated: Jul 4, 2026
06:08
Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Cellulase production by trichoderma harzianum in static and mixed solid-state fermentation reactors under nonaseptic
F Deschamps1, C Giuliano, M Asther
1Laboratoire de Biotechnologie, I.R.CH.A., B.P. No. 1, 91710 Vert-Le-Petit, France.
Biotechnology and Bioengineering
|September 1, 1985
Summary
This study optimized cellulase production from lignocellulosic materials using Trichoderma harzianum under nonaseptic solid-state fermentation. Optimal conditions yielded significant filter paper and endoglucanase activity, scalable to larger reactors.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Microbial Fermentation
Background:
- Lignocellulosic materials are abundant, renewable resources.
- Cellulases are crucial enzymes for biomass conversion.
- Solid-state fermentation (SSF) offers advantages for enzyme production.
Purpose of the Study:
- To optimize cellulase production from lignocellulosic substrates using Trichoderma harzianum.
- To investigate the impact of fermentation parameters on enzyme yield.
- To evaluate scalability of the process.
Main Methods:
- Solid-state fermentation (SSF) using a mixture of wheat straw and bran.
- Investigation of moisture content, pH, temperature, and aeration.
- Assay of filter paper activity and endoglucanase activity.
- Comparison of static and stirred tank reactor cultivation.
Main Results:
- Optimal moisture content of 74% and pH of 5.8 were determined.
- Maximum yields of 18 IU/g filter paper activity and 198 IU/g endoglucanase activity were achieved in 66 hours.
- Similar cellulase yields were obtained in a 65 L stirred tank reactor compared to static cultures.
Conclusions:
- Trichoderma harzianum efficiently produces cellulases from lignocellulosic materials under optimized SSF conditions.
- The fermentation process is robust and scalable for industrial applications.
- Optimized SSF parameters enhance enzyme production for biomass valorization.
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