[Improvement of the EIA kit for the detection of tetanus immunoglobulin in human sera]

For the program to eliminate tetanus neonatorum in this country, we have improved the sensitivity of enzyme immunoassay kit (EIA kit) prepared on 1987 for the detection of human tetanus immunoglobulin (TIG) by competitive principle. (Chinese J Microbiol Immunol 1987; 20: 269-278) Horse-radish peroxidase-conjugated to tetanus toxoid monoclonal antibody was involved in the new kit, and tetanus hybridoma clones were prepared by this laboratory. The lowest detectable TIG level is 0.05 IU/ml serum instead of 0.1 IU/ml serum. The dose-response curve and cut-off determination system of the new EIA kit are better than those of the original one. We proposed that the newly designed EIA kit could be used for understanding the TIG level in women who are in the age group for giving birth and in the tetanus vaccination group.