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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Updated: Jul 4, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

Intronic noncoding RNAs and splicing.

John W S Brown1, David F Marshall, Manuel Echeverria

  • 1Plant Sciences Division, University of Dundee at the Scottish Crop Research Institute (SCRI), Invergowrie, Dundee, DD2 5DA, UK. j.w.s.brown@dundee.ac.uk

Trends in Plant Science
|June 17, 2008
PubMed
Summary
This summary is machine-generated.

Small nucleolar RNAs (snoRNAs) and microRNAs (miRNAs) exhibit diverse gene organizations and maturation pathways. Intronic noncoding RNAs influence small RNA and host mRNA biogenesis, with processing and splicing balance regulating their levels.

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A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

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Last Updated: Jul 4, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Small noncoding RNAs (ncRNAs), including small nucleolar RNAs (snoRNAs) and microRNAs (miRNAs), display significant variation in gene organization across organisms.
  • This genomic diversity directly impacts the maturation pathways of these crucial regulatory molecules.

Purpose of the Study:

  • To explore the implications of noncoding RNA presence within introns on the biogenesis of both mature small RNAs and their host messenger RNA (mRNA).
  • To investigate how the interplay between processing/ribonucleoprotein assembly of intronic noncoding RNAs and the splicing machinery regulates ncRNA and host mRNA levels.

Main Methods:

  • Comparative analysis of gene organization and maturation pathways for snoRNAs and miRNAs.
  • Examination of the regulatory mechanisms governing intronic noncoding RNA processing and splicing.
  • Leveraging well-characterized snoRNA processing pathways in yeast, plants, and animals as a foundation.

Main Results:

  • Intronic noncoding RNAs significantly affect the production of mature small RNAs and host mRNAs.
  • The balance between intronic ncRNA processing/assembly and the splicing process is a key regulatory point for ncRNA and host mRNA abundance.
  • Established snoRNA processing mechanisms provide a framework for understanding intronic miRNA maturation.

Conclusions:

  • The spatial organization of ncRNAs within introns has profound effects on their own biogenesis and that of the host gene.
  • Understanding these regulatory dynamics is crucial for deciphering small RNA biology and gene expression control.
  • Further research into intronic plant miRNA processing, informed by snoRNA pathways, is warranted.