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Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Published on: August 10, 2022
Comparative protein-profile analysis of implanted versus non-implanted human blastocysts
Francisco Domínguez1, Blanca Gadea, Francisco J Esteban
1Fundacion IVI, Instituto Universitario IVI, Valencia University, C/Guadassuar 1 bajo, Valencia 46015, Spain. fdominguez@ivi.es
Human Reproduction (Oxford, England)
|June 17, 2008
Summary
Protein analysis of blastocyst culture media can non-invasively assess embryo viability. Specific protein changes in media from implanted embryos offer a new tool for selecting viable blastocysts in reproductive medicine.
Area of Science:
- Reproductive Medicine
- Embryology
- Proteomics
Background:
- Non-invasive embryo quality assessment is crucial in Reproductive Medicine.
- Protein profiling of blastocyst culture media may indicate embryo viability.
- This could aid in selecting embryos for transfer.
Purpose of the Study:
- To investigate if protein profiles in blastocyst culture media can differentiate between implanting and non-implanting embryos.
- To identify potential protein biomarkers for non-invasive embryo viability assessment.
Main Methods:
- Protein-array technology was used to analyze 24-hour conditioned media from blastocysts.
- Media from implanting and non-implanting blastocysts were compared to control media.
- Statistical and gene ontology functional analyses were performed.
Main Results:
- Soluble TNF receptor 1 and IL-10 increased; MSP-alpha, SCF, CXCL13, TRAILR3, and MIP-1beta decreased compared to control.
- CXCL13 and granulocyte-macrophage colony-stimulating factor were lower in media from implanting blastocysts.
- No proteins were significantly increased in media from implanting blastocysts.
Conclusions:
- Distinct protein profiles in culture media correlate with blastocyst implantation.
- These protein differences represent a novel non-invasive method for diagnosing blastocyst viability.
- This approach can enhance embryo selection in assisted reproductive technologies.
