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Related Experiment Video

Updated: Jul 4, 2026

Murine Ileocolic Bowel Resection with Primary Anastomosis
08:49

Murine Ileocolic Bowel Resection with Primary Anastomosis

Published on: October 29, 2014

Lessons learned: optimization of a murine small bowel resection model.

Janice A Taylor1, Colin A Martin, Rajalakshmi Nair

  • 1Division of Pediatric General and Thoracic Surgery, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, USA.

Journal of Pediatric Surgery
|June 19, 2008
PubMed
Summary

Maintaining mice in pathogen-free conditions and analyzing gene expression in adapted animals improves data reproducibility in small bowel resection (SBR) models. This enhances understanding of intestinal adaptation. Keywords: SBR, murine model, reproducibility, intestinal adaptation.

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Area of Science:

  • Gastroenterology
  • Animal Models
  • Molecular Biology

Background:

  • Reproducible data is crucial for murine disease models.
  • Small bowel resection (SBR) models are used to study intestinal adaptation.
  • Variability in SBR models can hinder research findings.

Purpose of the Study:

  • To identify factors contributing to data variability in a murine model of small bowel resection (SBR).
  • To optimize experimental conditions for enhanced reproducibility in SBR studies.

Main Methods:

  • Male C57Bl/6 mice were subjected to either sham surgery or 50% SBR.
  • Investigated effects of housing (pathogen-free vs. standard) and nutrition (powder vs. tube feeding).
  • Analyzed intestinal morphology and gene expression changes using regression and ANOVA.

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Related Experiment Videos

Last Updated: Jul 4, 2026

Murine Ileocolic Bowel Resection with Primary Anastomosis
08:49

Murine Ileocolic Bowel Resection with Primary Anastomosis

Published on: October 29, 2014

Postoperative Ileus Murine Model
04:26

Postoperative Ileus Murine Model

Published on: July 12, 2024

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice
06:40

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

Published on: February 10, 2023

Main Results:

  • Pathogen-free housing led to shorter ileal villi and greater villus growth post-SBR compared to standard housing.
  • Nutritional formulation did not significantly impact intestinal adaptation.
  • Gene expression analysis in adapted crypt cells showed more consistent and significant changes.

Conclusions:

  • Pathogen-free conditions and focusing gene expression analysis on morphologically adapted animals improve data sensitivity and specificity.
  • These refinements minimize experimental variability in SBR models.
  • Improved understanding of intestinal adaptation processes is achievable with these optimized methods.