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Updated: Jul 4, 2026

Luminescence Resonance Energy Transfer to Study Conformational Changes in Membrane Proteins Expressed in Mammalian Cells
Published on: September 16, 2014
Fluorescence resonance energy transfer based MCM-EDTA-Tb3+-MES sensor
Clint B Smith1, John E Anderson, Richard D Massaro
1US Army Engineer Research and Development Center, Topographic Engineering Center, Alexandria, VA 22315, USA. clint.b.smith@erdc.usace.army.mil
Abstract:
An in situ mesopourous surface imprinted polymeric (SIP) sensor was synthesized for a highly sensitive, selective, and kinetically faster detection of the high-vapor-pressure nerve gas surrogate methyl salicylate (MES). Visual detection occurred on the filtrate thin films at 25 pM. Other nerve gas surrogates, TP, DMP, DMMP, PMP, and 1,4-thioxane, were tested and showed a decrease in sensitivity compared to MES. In addition, 2,6-dipicolinic acid (DPA), a biological indicator, was also investigated and showed a decrease in sensitivity compared to MES. Finally, the detection plateau was reached at 40 s and at 1.5 x 10(-4) M from pH 6-11.

