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Diffusion01:12

Diffusion

Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
Diffusion01:21

Diffusion

Diffusion is a type of passive transport. In passive transport, a substance tends to move from an area of high concentration to an area of low concentration until the concentration is equal across the space. For example, take the diffusion of substances through the air. When someone opens a perfume bottle in a room filled with people, the perfume is at its highest concentration in the bottle and is at its lowest at the edges of the room. The perfume vapor will diffuse, or spread away, from the...
Behavior of Gas Molecules: Molecular Diffusion, Mean Free Path, and Effusion03:48

Behavior of Gas Molecules: Molecular Diffusion, Mean Free Path, and Effusion

Although gaseous molecules travel at tremendous speeds (hundreds of meters per second), they collide with other gaseous molecules and travel in many different directions before reaching the desired target. At room temperature, a gaseous molecule will experience billions of collisions per second. The mean free path is the average distance a molecule travels between collisions. The mean free path increases with decreasing pressure; in general, the mean free path for a gaseous molecule will be...
Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
Eulerian and Lagrangian Flow Descriptions01:22

Eulerian and Lagrangian Flow Descriptions

Fluid flow analysis is critical in many scientific and engineering disciplines, and two principal approaches are used to describe this flow: the Eulerian and Lagrangian methods. These methods offer different perspectives on monitoring and analyzing the motion of fluids, each with distinct advantages depending on the scenario.
The Eulerian method focuses on fixed points in space where fluid properties, such as velocity, pressure, and temperature, are observed as the fluid moves between these...
Diffusion on Chromatography Columns01:07

Diffusion on Chromatography Columns

In column chromatography, when an analyte is introduced as a narrow band at the top of the column, the solutes begin to separate and broaden, developing a Gaussian profile. This broadening occurs due to various factors, such as longitudinal diffusion.
Longitudinal diffusion occurs when the solute molecules in the mobile phase diffuse from the more concentrated center of the chromatographic band to the more dilute regions on either side, both towards and against the flow direction. This...

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Related Experiment Video

Updated: Jul 4, 2026

The Diffusion of Passive Tracers in Laminar Shear Flow
08:01

The Diffusion of Passive Tracers in Laminar Shear Flow

Published on: May 1, 2018

Measurement of Diffusion in Flowing Complex Fluids.

Edward F Leonard1, Christian P Aucoin, Edgar E Nanne

  • 1Department of Chemical Engineering Columbia University in the City of New York.

Colloids and Surfaces. A, Physicochemical and Engineering Aspects
|June 19, 2008
PubMed
Summary
This summary is machine-generated.

This study introduces a microfluidic device for measuring diffusion and particle movement in complex fluids. The device enables accurate diffusivity calculations under controlled flow conditions.

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Last Updated: Jul 4, 2026

The Diffusion of Passive Tracers in Laminar Shear Flow
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Area of Science:

  • Fluid dynamics
  • Physical chemistry
  • Biophysics

Background:

  • Measuring diffusion and particle dynamics in complex fluids is challenging.
  • Maintaining stable flow conditions is crucial for accurate measurements.
  • Existing methods may not be suitable for dynamic fluid environments.

Purpose of the Study:

  • To present a novel microfluidic device for quantifying solute and particle transport in flowing complex fluids.
  • To enable precise measurement of diffusivities in dynamic fluid systems.
  • To offer a method for calculating diffusion coefficients from observable concentration gradients.

Main Methods:

  • Development of a specialized microfluidic cell.
  • Implementation of controlled fluid flow within the microfluidic device.
  • Application of Loschmidt diffusion theory with short exposure times.
  • Analysis of concentration differences in outflowing streams.

Main Results:

  • The microfluidic device successfully measures solute and particle diffusion and migration.
  • The device maintains a desired flow state essential for accurate measurements.
  • Diffusivities can be calculated from concentration data using the presented method.

Conclusions:

  • The described microfluidic device offers a robust platform for studying transport phenomena in complex fluids.
  • The method provides a reliable way to determine diffusion coefficients under flow.
  • This technology advances the understanding of diffusion in dynamic and complex fluid systems.