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Using an Isolated Working Rat Heart Model to Test Donor Heart Preservation Strategies

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Postconditioning the isolated working rat heart.

Derick van Vuuren¹, Amanda Genis, Sonia Genade

¹Department of Biomedical Sciences, Division of Medical Physiology, Faculty of Health Sciences, University of Stellenbosch, P.O. Box 19063, Tygerberg, 7505, Republic of South Africa.

Cardiovascular Drugs and Therapy

|June 20, 2008

Summary

Postconditioning (postC) protects hearts from reperfusion injury. This study developed a cardioprotective postC protocol in a working heart model, showing infarct sparing effects sensitive to temperature.

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Area of Science:

Cardiovascular Physiology
Ischemia-Reperfusion Injury
Cardioprotection

Background:

Postconditioning (postC) is a cardioprotective strategy against reperfusion injury.
Its infarct-sparing effect in isolated working heart models remains unreported.
Developing an effective postC protocol for this model is crucial.

Purpose of the Study:

To develop a cardioprotective postconditioning protocol.
To investigate the infarct-sparing effect of postC in an isolated working heart model.
To assess the influence of temperature on postC efficacy.

Main Methods:

Rat hearts perfused retrogradely (Langendorff) or in working mode.
Global or regional ischemia followed by reperfusion.

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Postconditioning applied via brief ischemia/reperfusion cycles.

Infarct size measured; temperature varied (36.5°C vs. 37°C).

Main Results:

Postconditioning demonstrated an infarct-sparing effect at 37°C in both Langendorff and working heart models.
Significant infarct reduction (p<0.0001 in Langendorff, p<0.001 in working).
Functional preservation was observed only in the Langendorff group, not the working heart group.

Conclusions:

An infarct-sparing effect of postconditioning was achieved in the working heart model.
This effect was highly sensitive to temperature fluctuations.
Functional preservation was not associated with infarct sparing in the working heart model.