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Expression of human beta-defensins-1, 2 and 4 mRNA in human lung tumor tissue: a pilot study
T Shestakova1, E Zhuravel, L Bolgova
1R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology NAS of Ukraine, Kyiv, Ukraine.
Aim:
To analyze the patterns of human beta-defensin-1, 2, 4 (hBDs) expression in human lung tumors.
Materials And Methods:
Tissue samples of surgically resected human lung tumors (squamous cell carcinoma (SCC), n=10; adenocarcinoma (AC), n=10) paired with conditionally normal tissue samples were analyzed for expression of hBD-1, 2, 4 mRNA by semiquantitative RT-PCR.
Results:
In a number of studied lung cancer tissue samples, overexpression of defensin mRNA was registered: hBD-1 mRNA (50% of SCC and 60% AC), hBD-2 mRNA (60% of SCC and 50% of AC) or hBD-4 (40% of SCC and 20% AC). No correlation was detected between the levels of hBD-1, hBD-2 and hBD-4 mRNA and histological type, differentiation grade of the tumor, and the stage of the disease, as well as the content of hBD-2 peptide in blood serum of lung cancer patients.
Conclusion:
Human beta-defensins-1 and -2 are often up-regulated in human lung tumors.
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