Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Zygotic Development And Stem Cell Formation

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

Cell Culture

Cell Culture

Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Decoding the Apical-Basal Surfaceome of Colon Epithelial Cells via Side-Selective Biotinylation.

Biomolecules·2026

Same author

Molecular dynamics simulations elucidate the misfolding mechanisms of secretion-defective pancreatic lipase variants.

The FEBS journal·2026

Same author

Neuropilin-1-Targeted Cell-Penetrating Tandem Peptide-Drug Conjugate Exhibits Potent In Vivo Antiglioma and Antiangiogenic Activity.

Journal of medicinal chemistry·2026

Same author

[Physician, painter, soldier: Georges Chicotot (1855-1937)].

Orvosi hetilap·2026

Same author

Fermented and Unfermented Rooibos (<i>Aspalathus linearis</i>) Exhibit Selective Protection Against Hepatic Stress in Rats Exposed to Fumonisin B1.

Antioxidants (Basel, Switzerland)·2026

Same author

Effects of Dietary Supplementation with Black Soldier Fly Larvae (<i>Hermetia illucens</i>) Frass on Common Carp (<i>Cyprinus carpio</i>).

Animals : an open access journal from MDPI·2026

Same journal

Tau protein differentially affects Piezo1 and Kir2.1 channels in brain capillary endothelial cells.

Biophysical journal·2026

Same journal

Emergent Intercellular Junction Stability during Cyclic Tissue Loading.

Biophysical journal·2026

Same journal

Enhanced-Sampling Simulations Reveal Distinct Intermediates in SARS-CoV-2 FSE Pseudoknot Interconversion.

Biophysical journal·2026

Same journal

Structure-based simulations of the full Flock House virus capsid reveal pathways and energetics of an infection-critical peptide externalization event.

Biophysical journal·2026

Same journal

Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

Biophysical journal·2026

Same journal

Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

Biophysical journal·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

Multicellular sprouting in vitro.

Andras Szabo¹, Elod Mehes, Edina Kosa

¹Department of Biological Physics, Eotvos University, Budapest, Hungary.

Biophysical Journal

|June 24, 2008

Summary

This summary is machine-generated.

Elongated cells enhance and guide cell motility, influencing developmental processes like vasculogenesis. This study models this by modifying the cellular Potts model to favor adhesion to elongated cell surfaces.

More Related Videos

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Related Experiment Videos

Last Updated: Jul 4, 2026

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

Published on: May 11, 2021

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

2.5D Model for Ex Vivo Mechanical Characterization of Sprouting Angiogenesis in Living Tissue

Published on: February 28, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

Area of Science:

Cell Biology
Developmental Biology
Biophysics

Background:

Cell motility and guidance via cell-cell contacts are crucial for development and disease.
Vasculogenesis involves multicellular sprouts forming polygonal patterns, requiring endothelial cell supply.
In vitro studies show cell lines forming linear arrangements, mimicking vasculogenic sprouts.

Purpose of the Study:

To investigate how close contact with elongated cells influences cell motility.
To model the patterning process observed in multicellular linear arrangements.
To understand the role of cell shape in guided cell migration.

Main Methods:

Long-term videomicroscopy of in vitro cell cultures (C6 gliomas, 3T3 fibroblasts).
Augmentation of the cellular Potts model with a nonequilibrium interaction.
Modeling adhesion preferences based on cell shape (elongated vs. isotropic).

Main Results:

Evidence shows close contact with elongated cells enhances and guides cell motility.
The augmented cellular Potts model demonstrates patterning consistent with observed cell arrangements.
Surfaces of elongated cells are shown to be more preferred adhesion substrates than isotropic cells.

Conclusions:

Cell shape, specifically elongation, plays a significant role in guiding cell motility.
The modified cellular Potts model effectively simulates cell patterning driven by shape-dependent adhesion.
This research provides insights into the mechanisms underlying collective cell migration and tissue morphogenesis.