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Updated: Jul 4, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
pH but not hypoxia affects neonatal gene expression: relevance for housekeeping gene selection
Jill L Maron1, Michelle A Arya, Kimberly J Seefeld
1Department of Pediatrics, Tufts Medical Center, Boston, MA 02111, USA. jmaron@tufts-nemc.org
Objective:
To identify a candidate neonatal housekeeping gene and to determine the effects of pH and PaO(2) on the stability of newborn gene expression in physiologically hypoxic and acidotic newborn blood.
Methods:
Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) amplification was performed for four commonly used housekeeping genes (GAPDH, beta-actin, cyclophilin, 28S rRNA) on extracted RNA. Blood gas analyses determined pH and PaO(2) levels.
Results And Conclusions:
Beta-Actin was the least variable and GAPDH the most variable housekeeping gene studied. pH negatively correlated with gene expression levels. PaO(2) levels did not significantly affect gene expression. These results inform selection of housekeeping genes for neonatal mRNA research.
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