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Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance
Published on: January 3, 2012
Self-assembled monolayer for toxicant detection using nucleic acid sensor based on surface plasmon resonance
Pratima R Solanki1, Nirmal Prabhakar, M K Pandey
1Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr K. S. Krishnan Road, New Delhi, 110012, India.
Biomedical Microdevices
|June 25, 2008
Summary
This study developed a novel biosensor using immobilized double-stranded calf thymus deoxyribonucleic acid (dsCT-DNA) on gold substrates. The biosensor successfully detected cypermethrin at low concentrations using surface plasmon resonance (SPR) technology.
Area of Science:
- Biomolecular Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Developing sensitive and specific biosensors is crucial for environmental monitoring and public health.
- Surface plasmon resonance (SPR) and electrochemical techniques offer label-free detection methods for biomolecular interactions.
- Immobilization of deoxyribonucleic acid (DNA) on transducer surfaces is a key strategy for fabricating DNA-based biosensors.
Purpose of the Study:
- To fabricate a novel surface plasmon resonance (SPR) based biosensing device for detecting cypermethrin.
- To covalently immobilize double-stranded calf thymus deoxyribonucleic acid (dsCT-DNA) onto a gold substrate modified with a beta-mercaptoethanol (MCE) self-assembled monolayer (SAM).
- To characterize the fabricated bioelectrode and evaluate its performance in detecting cypermethrin.
Main Methods:
- Covalent immobilization of dsCT-DNA onto MCE-SAM/Au using carbodiimide chemistry.
- Characterization of the bioelectrode using electrochemical impedance spectroscopy, cyclic voltammetry, contact angle measurements, and atomic force microscopy.
- Detection of cypermethrin using electrochemical and SPR techniques.
Main Results:
- Successful fabrication and characterization of the dsCT-DNA-MCE-SAM/Au bioelectrode.
- Demonstrated detection of cypermethrin at a concentration of 0.0005 ppm.
- Proposed a mechanism for the interaction between cypermethrin and the immobilized dsCT-DNA.
Conclusions:
- The developed dsCT-DNA-based SPR biosensor is effective for sensitive cypermethrin detection.
- The immobilization strategy and characterization methods provide a robust platform for biosensor development.
- This approach holds promise for environmental monitoring of pesticide contamination.
