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Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Extracellular functions of thioredoxin.
1Thioredoxin Project, Department of Experimental Therapeutics, Translational Research Center, Kyoto University Hospital, 54 Shogoin Kawaharacho Sakyo, Kyoto 606-8507, Japan.
Novartis Foundation Symposium
|June 26, 2008
Summary
Thioredoxin (TRX) protein reduces oxidative stress and inflammation. This study shows TRX inhibits macrophage migration inhibitory factor (MIF), suggesting recombinant TRX as a potential therapy for inflammatory diseases.
Area of Science:
- Biochemistry
- Immunology
- Molecular Biology
Background:
- Thioredoxin (TRX) is a vital protein involved in redox homeostasis.
- TRX possesses a conserved active site (-Cys-Xxx-Xxx-Cys-) and plays roles in cellular activation and apoptosis.
- TRX is released from cells under oxidative stress, serving as a biomarker and exhibiting extracellular anti-inflammatory properties.
Purpose of the Study:
- To investigate the anti-inflammatory mechanisms of Thioredoxin (TRX).
- To explore the relationship between TRX and macrophage migration inhibitory factor (MIF).
- To evaluate the therapeutic potential of recombinant TRX in inflammatory conditions.
Main Methods:
- Analysis of TRX's redox-active properties.
- Investigating TRX's role in oxidative stress response.
- Assessing TRX's effects on inflammatory markers, including MIF expression and release.
Main Results:
- Thioredoxin (TRX) effectively scavenges reactive oxygen species.
- Extracellular TRX demonstrates anti-inflammatory effects by inhibiting neutrophil extravasation.
- TRX was found to inhibit the expression and release of the pro-inflammatory cytokine macrophage migration inhibitory factor (MIF).
Conclusions:
- Circulating TRX levels are indicative of oxidative stress.
- TRX exhibits significant anti-inflammatory actions, partly through MIF modulation.
- Recombinant TRX administration presents a promising therapeutic strategy for acute inflammatory disorders.
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