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Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology

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Periodic optimization of continuous microbial growth processes.

E M Abulesz¹, G Lyberatos

¹Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611.

Biotechnology and Bioengineering

|June 1, 1987

Summary

Periodic operation of bioreactors, especially with time delays, can outperform steady-state conditions for biomass production. This dynamic approach, using the pi-criterion method, enhances average protein productivity in fermentation processes.

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Area of Science:

Biochemical Engineering
Bioprocess Optimization
Fermentation Technology

Background:

Continuous bioreactor operation typically assumes steady-state conditions for optimal performance.
However, the dynamic behavior of bioreactors, including time delays, can significantly impact process efficiency.
The suitability of steady-state operation versus dynamic control strategies requires further investigation.

Purpose of the Study:

To evaluate whether periodic variation of the dilution rate can enhance continuous fermentation processes.
To determine if dynamic operation is superior to steady-state operation for biomass production in chemostats.
To assess the impact of periodic operation on average protein productivity using a structured model.

Main Methods:

Application of the pi-criterion method to analyze bioreactor dynamics.
Mathematical modeling using Williams' structured model to simulate fermentation processes.
Comparison of performance metrics between steady-state and periodic (cycling) operation modes.

Main Results:

The presence of time delay in chemostat dynamics makes periodic operation superior to steady-state operation for biomass production.
Periodic variation of the dilution rate was found to enhance the overall performance of continuous fermentation.
Williams' structured model confirmed that cycling improves average protein productivity.

Conclusions:

Periodic operation, particularly in the presence of time delays, offers significant advantages over steady-state operation for bioreactors.
Dynamic control strategies, such as varying the dilution rate, can optimize biomass production and protein yield.
The pi-criterion method is a valuable tool for identifying optimal operating modes in bioprocesses.