[Stability of lysosomal membranes with different protocols of freezing--thawing]

The non-sedimentary activity of the liver hydrolases (acid RNase, DNase, phosphatase and cathepsins) of albino rats was studied at different freezing-thawing programmes (rapid and two-staged). It is found that the non-sedimentary activity of hydrolases increases in case of all freezing-thawing programmes. The lowest activity is observed at two-staged programmes, and after rapid freezing and rapid hawing of lysosomes.