Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Familial florid cemento-osseous dysplasia with ANO5 mutation: a case report].

Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology·2026
Same author

Penicillin cross-sensitivity in patients with confirmed peri-operative allergic hypersensitivity reactions to cefazolin: a retrospective observational study.

Anaesthesia·2023
Same author

Retrospective observational study of the incidence of peri-operative allergic hypersensitivity reactions to cefazolin.

Anaesthesia·2023
Same author

CRISPR-based gene editing enables <i>FOXP3</i> gene repair in IPEX patient cells.

Science advances·2020
Same author

CXCR3 ligands in pleural fluid as markers for the diagnosis of tuberculous pleural effusion.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2018
Same author

Early discoid lupus erythematosus protects against renal disease in patients with systemic lupus erythematosus: longitudinal data from a large Latin American cohort.

Lupus·2016

Related Experiment Video

Updated: Jul 4, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Food surface texture measurement using reflective confocal laser scanning microscopy.

S Sheen1, G Bao, P Cooke

  • 1Eastern Regional Research Center, Microbial Food Safety Research Unit, Agricultural Research Service, U.S. Dept. of Agriculture, 600 E. Mermaid Lane, Wyndmoor, PA 19038, USA. shiowshuh.sheen@ars.usda.gov

Journal of Food Science
|June 26, 2008
PubMed
Summary

Confocal laser scanning microscopy accurately measured the surface roughness of sliced foods like ham, salami, and cheese. This technique offers a reliable method for food surface texture analysis.

More Related Videos

Isolation and Biophysical Study of Fruit Cuticles
15:53

Isolation and Biophysical Study of Fruit Cuticles

Published on: March 30, 2012

Laser Micromachining for Polymer Surface Topography Design
05:49

Laser Micromachining for Polymer Surface Topography Design

Published on: September 19, 2025

Related Experiment Videos

Last Updated: Jul 4, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells
09:20

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Isolation and Biophysical Study of Fruit Cuticles
15:53

Isolation and Biophysical Study of Fruit Cuticles

Published on: March 30, 2012

Laser Micromachining for Polymer Surface Topography Design
05:49

Laser Micromachining for Polymer Surface Topography Design

Published on: September 19, 2025

Area of Science:

  • Food science and technology
  • Materials science
  • Microscopy

Background:

  • Characterizing food surface texture is crucial for understanding properties like microbial adhesion and transfer.
  • Standardized methods are needed for accurate and reproducible surface roughness measurements in food products.

Purpose of the Study:

  • To adapt and validate Confocal Laser Scanning Microscopy (CLSM) for quantifying the surface texture of sliced food items.
  • To establish reliable instrument settings using calibrated sandpapers for food surface analysis.

Main Methods:

  • Utilized Confocal Laser Scanning Microscopy (CLSM) in reflection mode to analyze surface topography.
  • Employed sandpapers (grit 150-600) as height references, verified by Scanning Electron Microscopy (SEM), to calibrate CLSM settings.
  • Measured surface texture parameters including Ra, Rs, Pa, and Pq for mechanically sliced ham, salami, and cheese.

Main Results:

  • CLSM measurements of sandpaper surface variations closely matched standard grit particle sizes.
  • Surface roughness values for sliced ham, salami, and cheese were found to be comparable to the dimensions of the calibrated sandpapers.
  • The study successfully quantified key surface texture parameters for the tested food samples.

Conclusions:

  • CLSM provides a viable method for characterizing the surface texture of sliced foods.
  • This technique can be applied to further research on food surface properties and their influence on microbial adhesion and transfer.
  • The established calibration method ensures accuracy in food surface texture analysis.