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Updated: Jul 4, 2026

Characterization of pH-Dependent Reversible Self-Assembly of Amyloid Beta 1-40-Coated Gold Colloids
Published on: March 21, 2025
Assembly of polypeptide-functionalized gold nanoparticles through a heteroassociation- and folding-dependent bridging
Daniel Aili1, Karin Enander, Lars Baltzer
1Division of Sensor Science and Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, Linköping, Sweden.
Abstract:
Gold nanoparticles were functionalized with a synthetic polypeptide, de novo-designed to associate with a charge complementary linker polypeptide in a folding-dependent manner. A heterotrimeric complex that folds into two disulphide-linked four-helix bundles is formed when the linker polypeptide associates with two of the immobilized peptides. The heterotrimer forms in between separate particles and induces a rapid and extensive aggregation with a well-defined interparticle spacing. The aggregated particles are redispersed when the disulphide bridge in the linker polypeptide is reduced.
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