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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
Published on: March 27, 2020
TEAD mediates YAP-dependent gene induction and growth control
1Department of Pharmacology and Moores Cancer Center, University of California at San Diego, La Jolla, California 92093, USA.
Genes & Development
|June 27, 2008
Summary
The TEAD family transcription factors are essential for YAP-mediated gene expression, cell growth, and oncogenic transformation. This study reveals TEAD as a key component of the Hippo pathway, crucial for YAP
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- The YAP transcription coactivator is implicated as an oncogene and amplified in human cancers.
- YAP is phosphorylated and inhibited by the Hippo tumor suppressor pathway.
Purpose of the Study:
- To investigate the role of TEAD family transcription factors in mediating YAP-dependent gene expression and biological functions.
- To identify direct YAP target genes involved in cell growth.
- To explore the conservation of the YAP-TEAD functional relationship in Drosophila.
Main Methods:
- Demonstration of TEAD's essential role in mediating YAP-dependent gene expression.
- Assessment of TEAD's requirement for YAP-induced cell growth, oncogenic transformation, and epithelial-mesenchymal transition.
- Identification of CTGF as a direct YAP target gene.
- Comparative analysis of YAP and TEAD homologs in Drosophila.
Main Results:
- TEAD family transcription factors are essential for YAP-dependent gene expression.
- TEAD is required for YAP-induced cell growth, oncogenic transformation, and epithelial-mesenchymal transition.
- CTGF is identified as a direct YAP target gene crucial for cell growth.
- The functional relationship between YAP and TEAD is conserved in Drosophila (Yki and Scalloped).
Conclusions:
- TEAD family transcription factors are essential mediators of YAP's biological functions.
- TEAD plays critical roles in YAP-induced oncogenesis and cell proliferation.
- TEAD represents a new component of the Hippo pathway, essential for mediating YAP's functions.
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