Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Production of Organic Acids01:25

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...
Production of Alcohol01:27

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter.

Applied microbiology and biotechnology·2000
Same author

Polysaccharide concentration and molecular weight effects on the oxygen mass transfer in a reciprocating plate bioreactor.

Biotechnology and bioengineering·1996
Same author

Measuring kLa with randomly pulsed dynamic method.

Biotechnology and bioengineering·1991
Same author

Effect of growth conditions and trehalose content on cryotolerance of bakers' yeast in frozen doughs.

Applied and environmental microbiology·1989
Same author

Improvement of ethanol fermentation under hyperbaric conditions.

Biotechnology and bioengineering·1989
Same author

Biosynthesis of pullulan using immobilized Aureobasidium pullulans cells.

Biotechnology and bioengineering·1989
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

Related Experiment Video

Updated: Jul 4, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Pullulan from peat hydrolyzate fermentation kinetics.

J M Boa1, A Leduy

  • 1Department of Chemical Engineering, Laval University, Sainte-Foy, Québec, Canada G1K 7P4.

Biotechnology and Bioengineering
|September 1, 1987
PubMed
Summary
This summary is machine-generated.

This study optimized pullulan fermentation kinetics using the Luedeking-Piret model. Fed-batch fermentation significantly enhances polysaccharide productivity compared to batch or continuous methods.

More Related Videos

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
16:33

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes

Published on: December 12, 2013

Related Experiment Videos

Last Updated: Jul 4, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
14:53

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

Published on: October 24, 2016

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock
07:24

Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock

Published on: June 29, 2017

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes
16:33

Methods for Facilitating Microbial Growth on Pulp Mill Waste Streams and Characterization of the Biodegradation Potential of Cultured Microbes

Published on: December 12, 2013

Area of Science:

  • Biotechnology
  • Biochemical Engineering
  • Microbial Fermentation

Background:

  • Pullulan, a polysaccharide, is produced via microbial fermentation.
  • Optimizing fermentation conditions is crucial for maximizing pullulan yield and productivity.
  • Peat hydrolyzate offers a potential sustainable substrate for fermentation processes.

Purpose of the Study:

  • To analyze pullulan fermentation kinetics using the Luedeking-Piret equation.
  • To compare the efficiency of batch, continuous, and fed-batch fermentation modes.
  • To determine optimal conditions for enhanced polysaccharide production.

Main Methods:

  • Application of the Luedeking-Piret model to fit kinetic data.
  • Fermentation experiments conducted in batch, continuous, and constant-feed fed-batch modes.
  • Variations in kinetic parameters (m, n, alpha, beta) analyzed under different conditions (aeration, agitation, temperature, feed rates).

Main Results:

  • Kinetic parameters varied with fermentation conditions in batch mode.
  • Polysaccharide synthesis was growth-associated in all tested modes.
  • Fed-batch fermentation demonstrated higher overall polysaccharide productivity compared to batch and continuous modes.

Conclusions:

  • Fed-batch fermentation, particularly when initiated batchwise followed by a constant feed rate, is superior for pullulan production.
  • Optimized fed-batch strategies significantly increase polysaccharide productivity (g pullulan/L h).
  • The Luedeking-Piret model effectively describes pullulan fermentation kinetics under various conditions.