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The effects of Tordon 202c exposure on urethan-induced lung adenoma formation in female CD-1 mice
S L Adams1, S T Horvat, A E Irwin
1Department of Veterinary Physiological Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.
Abstract:
Female CD-1 mice were exposed to Tordon 202c, a herbicide containing 2,4-dichlorophenoxyacetic acid and picloram, in the drinking water for 15 w at concentrations ranging from 0 to 0.3% of the product formulation. After 3 w of the 15-w treatment period, the mice received 1.5 mg/g urethan ip. Pulmonary adenoma production was evaluated 12 w later. Tordon 202c exposure produced a dose-dependent increase in tumor number, but had no effect on tumor size. Urethan-induced sleeping times which reflected the rate of urethan metabolism or excretion were altered, but a specific dose-related effect which could be correlated with tumor production was not observed. This suggests that Tordon 202c exposure influences adenoma formation by immunological mechanisms rather than by causing indirect effects on urethan metabolism or excretion.
Insights
Exposure to the herbicide Tordon 202c, containing 2,4-dichlorophenoxyacetic acid and picloram, increased pulmonary adenoma formation in mice. This suggests Tordon 202c influences tumor development through immunological mechanisms.
Area of Science:
- Toxicology
- Carcinogenesis
- Environmental Health
Background:
- The herbicide Tordon 202c contains 2,4-dichlorophenoxyacetic acid and picloram.
- Understanding the carcinogenic potential of herbicides is crucial for public health.
- Previous studies have not fully elucidated the mechanisms by which Tordon 202c may affect tumor development.
Purpose of the Study:
- To investigate the effect of Tordon 202c exposure on pulmonary adenoma production in mice.
- To explore the potential mechanisms, including immunological effects and alterations in chemical metabolism, underlying Tordon 202c's influence on tumor formation.
Main Methods:
- Female CD-1 mice were administered Tordon 202c in drinking water for 15 weeks.
- Urethane was administered intraperitoneally at 3 weeks.
- Pulmonary adenoma incidence and size were evaluated 12 weeks after urethane administration.
- Urethane-induced sleeping times were measured to assess metabolism/excretion rates.
Main Results:
- Tordon 202c exposure resulted in a dose-dependent increase in the number of pulmonary adenomas.
- Tumor size was not significantly affected by Tordon 202c exposure.
- While urethane-induced sleeping times were altered, no clear dose-related correlation with tumor production was observed.
Conclusions:
- Tordon 202c exposure promotes pulmonary adenoma formation in a dose-dependent manner.
- The findings suggest that Tordon 202c influences adenoma development primarily through immunological mechanisms.
- The herbicide's effect is unlikely to be mediated by significant alterations in urethane metabolism or excretion.