A syngeneic hepatocellular carcinoma model rapidly and simply prepared using a hydrodynamics-based procedure

A rapid and simple method to deliver tumor cells into the liver by intravenous injection of a large volume of tumor cell solution at a high velocity has been developed for use in the preparation of hepatic cancer mouse models. With this hydrodynamics-based procedure, there was a 100% tumor occurrence in the liver and lungs of mice at 2 and 3 weeks, respectively. In contrast, mice injected using a non-hydrodynamics-based system showed no tumor occurrence in the liver 3 weeks after injection, although tumors were present in the lungs. The technique may be useful in the rapid development of hepatic cancer mouse models and in reducing animal mortality during model preparation.