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Related Experiment Video

Updated: Jul 4, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

Published on: May 1, 2015

Growth hormone promotes lymphangiogenesis.

Nadja Erika Banziger-Tobler¹, Cornelia Halin, Kentaro Kajiya

¹Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Wolfgang-Pauli-Str. 10, HCI H303, CH-8093 Zurich, Switzerland.

The American Journal of Pathology

|June 28, 2008

Summary

Growth hormone promotes lymphatic vessel growth and survival. This study identifies growth hormone as a novel lymphangiogenic factor, crucial for understanding lymphatic system functions in inflammation and cancer.

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Last Updated: Jul 4, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting

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Published on: May 1, 2015

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Generation of Lymph Node-fat Pad Chimeras for the Study of Lymph Node Stromal Cell Origin

Published on: December 16, 2013

Area of Science:

Endocrinology
Molecular Biology
Vascular Biology

Background:

The lymphatic system is vital in inflammation and cancer, but its molecular regulation is unclear.
Understanding lymphatic endothelial cell (LEC) behavior is key to targeting lymphatic system dysfunctions.

Purpose of the Study:

To investigate the role of growth hormone (GH) in regulating lymphatic endothelial cells.
To identify novel molecular mechanisms governing lymphangiogenesis.

Main Methods:

Comparative transcriptional profiling of LECs and blood vascular endothelial cells (BECs).
Quantitative real-time reverse transcriptase-polymerase chain reaction (qRT-PCR) and Western blot analysis.
In vitro functional assays (proliferation, migration, tube formation, apoptosis) and in vivo studies using GH-transgenic mice and wild-type mice.

Main Results:

Growth hormone receptor (GHR) is significantly upregulated in LECs compared to BECs.
GH stimulates LEC proliferation, migration, sprouting, and tube formation independently of VEGFRs.
GH inhibits LEC apoptosis and promotes lymphatic vessel growth in vivo, particularly in wound healing models.

Conclusions:

Growth hormone acts as a novel and potent lymphangiogenic factor.
Targeting GH signaling may offer therapeutic strategies for diseases involving lymphatic system dysfunction.