Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Electrochemical Detection of Organophosphates Using Dual-Modal Enzyme-Based Biosensors.

Analytical chemistry·2026

Same author

Optical Analysis of Cyclic Voltammetry of Ferrocenemethanol: A Comparative Study of SPR and LSPR.

Langmuir : the ACS journal of surfaces and colloids·2026

Same author

Smart Catheters for Diagnosis, Monitoring, and Therapy.

Advanced healthcare materials·2025

Same author

Advances in biosensors for diagnosis of Alzheimer's and Parkinson's diseases.

Biosensors & bioelectronics·2025

Same author

Author Correction: Bioinstructive implantable scaffolds for rapid in vivo manufacture and release of CAR-T cells.

Nature biotechnology·2025

Same author

Overcoming the effects of fluid shear stress in ovarian cancer cell lines: Doxorubicin alone or photodynamic priming to target platinum resistance.

Photochemistry and photobiology·2024

Same journal

Tunable self-assembling cellular microarray for single-neutrophil vital and suicidal extracellular traps.

Lab on a chip·2026

Same journal

Precise programmable tumor cell subpopulation sorting <i>via</i> an electromagnetic microfluidic platform.

Lab on a chip·2026

Same journal

Bridging dimensions: combining one- and two-photon 3D printing for microfluidic device fabrication.

Lab on a chip·2026

Same journal

Microfluidic rare cell analysis beyond counting: workflow design from enrichment to multi-omics.

Lab on a chip·2026

Same journal

A sperm racetrack to separate sperm by swim speed.

Lab on a chip·2026

Same journal

Controlled encapsulation and droplet size prediction in two-step microfluidic double emulsions.

Lab on a chip·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 4, 2026

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

Two simple and rugged designs for creating microfluidic sheath flow.

Peter B Howell¹, Joel P Golden, Lisa R Hilliard

¹Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.

|June 28, 2008

Summary

This summary is machine-generated.

This study introduces a simple microfluidic design for 2-dimensional hydrodynamic focusing. The novel approach achieves full sample stream sheathing with a single input, preventing sample loss and simplifying fabrication.

More Related Videos

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

Related Experiment Videos

Last Updated: Jul 4, 2026

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Microfluidic Fabrication of Polymeric and Biohybrid Fibers with Predesigned Size and Shape

Published on: January 8, 2014

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

High Throughput Microfluidic Rapid and Low Cost Prototyping Packaging Methods

Published on: December 23, 2013

Area of Science:

Microfluidics
Biotechnology
Chemical Engineering

Background:

Traditional microfluidic sheath flow systems often fail to fully enclose sample streams, leading to sample adsorption.
Existing methods for complete sample sheathing typically involve complex multi-inlet designs requiring precise flow balancing and alignment.

Purpose of the Study:

To present a novel, simplified microfluidic design for achieving 2-dimensional hydrodynamic focusing.
To demonstrate a method for complete sheathing of a sample stream using minimal inputs.
To offer a design that mitigates sample adsorption and allows for fluid separation.

Main Methods:

Development of a simple microfluidic chip architecture for hydrodynamic focusing.
Fabrication using poly(dimethylsiloxane) (PDMS) via soft lithography.
Fabrication using poly(methyl methacrylate) (PMMA) via micromilling and laser ablation.

Main Results:

Successful demonstration of 2-dimensional hydrodynamic focusing with full sample stream sheathing.
Achieved complete sheathing using only one sheath fluid input, simplifying system operation.
The design allows for subsequent separation of sample and sheath fluids for recapture or reuse.

Conclusions:

The proposed microfluidic design offers an effective and simplified solution for 2-dimensional hydrodynamic focusing.
This approach minimizes sample loss due to adsorption and reduces fabrication complexity.
The ability to separate fluids enhances the utility of microfluidic devices in various applications.