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Updated: Jul 4, 2026

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
Published on: January 23, 2019
Comparison of free and immobilized Pichia pastoris cells for conversion of ethanol to acetaldehyde
1Division of Biological Sciences, National Research Council of Canada, 100 Sussex Drive, Ottawa, Ontario, Canada, K1A ORG.
Abstract:
Free and immobilized cells of Pichia pastoris were used to convert ethanol to acetaldehyde in small-scale batch reactors. Immobilized cells were less active than free cells (V(max) free = 7.81 g/L h, V(max) immobilized = 3.17 g/L h) due to a number of factors including end product inhibition and diffusional limitations. Immobilized cells were more resistant to heat denaturation both in the presence and absence of ethanol. Immobilized cells retained more of their activity during repeated batch cycles than did free cells.
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