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Updated: Jul 4, 2026
11:56
A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
Published on: April 11, 2014
Potentiometric measurement of ascorbate by using a solvent polymeric membrane electrode.
Huimin Guo1, Tanji Yin, Qingmei Su
1Laboratory of Environmental Chemistry, Yantai Institute of Coastal Zone Research for Sustainable Development, Chinese Academy of Sciences, P.O. Box 1488, Yantai 264003, PR China.
Talanta
|July 1, 2008
Summary
A new potentiometric method enables sensitive detection of ascorbate using a specialized membrane electrode. This approach offers continuous and reversible measurement of vitamin C levels.
Area of Science:
- Analytical Chemistry
- Electrochemistry
- Biosensing
Background:
- Ascorbate (vitamin C) determination is crucial in various fields.
- Existing methods for ascorbate detection can be complex or lack continuous monitoring capabilities.
- Development of novel electrochemical sensors is needed for efficient ascorbate analysis.
Purpose of the Study:
- To introduce a novel potentiometric method for ascorbate determination.
- To utilize a ligand-free tridodecylmethylammonium chloride (TDMAC)-based polymeric membrane ion-selective electrode (ISE).
- To enable continuous and reversible detection of ascorbate.
Main Methods:
- A potentiometric method employing a TDMAC-based polymeric membrane ISE.
- Continuous release of permanganate from an inner reference solution for ascorbate oxidation.
- Monitoring of ISE potential changes due to permanganate activity at the sample-membrane interface.
Main Results:
- The proposed ISE demonstrated a potentiometric response to ascorbate.
- Continuous and reversible detection of ascorbate was achieved.
- The method detected ascorbate in 0.1M NaCl over a concentration range of 1.0 x 10(-6) to 1.0 x 10(-3)M.
- A low detection limit of 2.2 x 10(-7)M for ascorbate was established.
Conclusions:
- The novel potentiometric method provides a sensitive and reliable way to determine ascorbate.
- The TDMAC-based membrane electrode is suitable for continuous and reversible ascorbate monitoring.
- This method offers a promising alternative for ascorbate analysis in various applications.
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