Quantification of components in overlapping peaks from capillary electrophoresis by using continues wavelet transform...

Area of Science:

• Analytical Chemistry
• Chromatography
• Signal Processing

Background:

• Capillary Electrophoresis (CE) often produces overlapping peaks, complicating accurate compound identification and quantification.
• Existing methods for resolving overlapping peaks may struggle with noisy experimental data.

Purpose of the Study:

• To evaluate the effectiveness of Continues Wavelet Transform (CWT) for resolving overlapping peaks in CE.
• To determine the composition of benzoic acid and salicylic acid in a traditional Chinese patent medicine using CWT.

Main Methods:

• Experimental signals from CE were transformed into wavelet coefficients using CWT.
• The CWT method was applied to resolve overlapping peaks of benzoic acid and salicylic acid.
• Quantification results were compared with those obtained using the numerical differentiation method.

Main Results:

• CWT successfully resolved overlapping peaks from CE, enabling easier analysis.
• The method accurately determined the composition of benzoic acid and salicylic acid in Zuguangsan.
• CWT demonstrated superior performance compared to the numerical differentiation method, especially in processing noisy signals.

Conclusions:

• Continues Wavelet Transform (CWT) is a practical and effective approach for resolving overlapping peaks in CE.
• CWT provides accurate quantitative determination of coeluted compounds in CE, even with noisy data.
• CWT offers an advantage over numerical differentiation for processing complex CE electropherograms.