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Scale-Up Processes01:14

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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Bioreactor Design and Operational System01:29

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Operation of a Benchtop Bioreactor
12:54

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Published on: September 12, 2013

Optimization of batch fermentor sterilization.

V Singh1, W Hensler, R Fuchs

  • 1Schering Corporation, 1011 Morris Ave, Union, New Jersey 07083, USA.

Biotechnology and Bioengineering
|January 25, 1989
PubMed
Summary
This summary is machine-generated.

This study introduces a calculation method to optimize batch sterilization in large fermentors, minimizing nutrient damage and improving antibiotic yields. This approach aids in scaling up sterilization processes efficiently.

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Area of Science:

  • Bioprocess Engineering
  • Chemical Engineering
  • Industrial Microbiology

Background:

  • Sterilization is critical for large-scale fermentations to prevent contamination.
  • Optimizing sterilization cycles is essential to balance microbial control with nutrient integrity.
  • Scale-up of sterilization parameters can significantly impact product yield and process economics.

Purpose of the Study:

  • To present a calculation procedure for optimizing batch sterilization cycles in large-scale fermentors.
  • To determine optimal sterilization temperature and hold-time to minimize nutrient damage.
  • To evaluate the importance of scaling up sterilization conditions for product yield.

Main Methods:

  • Development of a calculation procedure for sterilization optimization.
  • Application of the method for determining temperature and hold-time.
  • Utilizing "scaledown" experiments to assess sterilization parameters.
  • Systematic evaluation of sterilization conditions' impact on product yield.

Main Results:

  • The procedure successfully determines optimal sterilization conditions for large fermentors.
  • Significant yield improvements were observed in a 1.2 x 10(5) L antibiotic fermentation using the presented techniques.
  • The method confirmed the importance of scale-up considerations for sterilization in specific processes.

Conclusions:

  • The presented calculation procedure is effective for optimizing and scaling up batch sterilization cycles.
  • Minimizing nutrient damage during sterilization leads to improved product yields.
  • This methodology provides a systematic approach to evaluate and implement efficient sterilization strategies in industrial fermentations.