Related Experiment Video
Updated: Jul 4, 2026
Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
Published on: April 22, 2016
Bioactive polymers. 56: urease immobilization on carboxymethylcellulose
S Dumitriu1, M Popa, V Artenie
1Polytechnic Institute of Jassy, Department of Organic and Macromolecular Chemistry and Technology, 23 August Street No. 11 A, 6600 Jassy, Romania.
Abstract:
This article reports on the reaction of urease immobilization through its covalent bonding on carboxymethylcellulose. The reaction is activated by dicyclohexylcarbodiimide. The coupling reaction is influenced by the enzyme-support and activator-support ratios, as well as by duration. Starting from a rotating, composed experimental program of the second order, the function correlating the activity of the immobilized enzyme with the reaction parameters is established. Immobilized urease exhibits thermal stability higher than that of free enzyme, regarding both pH and the inhibiting action of some metal ions or organic substances. The stability over time of the immobilized urease is high, its enzymatic activity being maintained at over 85% of the initial value three months after synthesis.
