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[Determination of 5-hydroxymethyl furfuraldehyde in different processed products of polygonatum]
Yun Yang1, Min Xu, Yun-xia Feng
1Henan College of Traditional Chinese Medicine, Zhengzhou, China. yyun@china.com.cn
Objective:
By finding the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Polygonatum for different time, this provides basis for the technology of processing Polygonatum and quality standards of its prepared pieces.
Methods:
The testing solution was acquired by ultrasonic extraction, HPLC was used to determine the contents. The chromatographic conditions: Agilent HC-C18 column (5 microm, 250 mm x 4.6 mm). The mobile phase: methanol-water (15:85) at the flow rate of 1 ml/min. The determination wave length was 284 nm.
Results:
All kinds of processed Polygonatum had 5-Hydroxymethyl furfuraldethyde in different degrees, contents in Polygonatum kept relatively stable before 30 hours, but contents rose rapidly after 30 hours, contents decreased as continuing heating.
Conclusion:
The result can provide useful references to quality control and standard porcessing condition of Polygonatum.
