Related Experiment Video
Updated: Jul 4, 2026

Using Coculture to Detect Chemically Mediated Interspecies Interactions
Published on: October 31, 2013
[The analysis of promoters and the effective expression of exogenous proteins in a cold-adapted bacterium]
Yunlin Wei1, Xiuling Ji, Lianbing Lin
1Biotechnology Research Center of Kunming University of Science and Technology, Kunming 650224, China. weiyunlin@yahoo.com.cn
Abstract:
Based on the constructed promoter probe vectors that could replicate both in E. coli and in a cold-adapted bacterium, several candidate promoters were isolated and their activities were evaluated by RT-PCR. The transcription initiation sites and core sequence of promoters were determined by primer extension analysis. A low-temperature expression vector was constructed by using the strongest promoter and a thermolabile alpha-amylase gene was successfully overproduced under control of this promoter at low temperature (7 degrees C), while the secreted alpha-amylase amounted up to 35% of the total extracellular proteins. The expression system is expected to be useful for the production of thermolabile exogenous proteins at low temperatures.
Related Concept Videos
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
Stringent Response in E. coli
Factors Influencing Microbial Growth: Temperature
Reporter Genes
Commonly used reporter...
Gene Regulation During Sporulation
Coordination of Gene Expression Processes in Bacteria

