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Improved procedure for the second-derivative spectrophotometric analysis of carboxyhaemoglobin
1Department of Clinical Chemistry, Streekziekenhuis Midden-Twente, Hengelo, The Netherlands.
Annals of Clinical Biochemistry
|March 1, 1991
Abstract:
We describe a modification of the second-derivative spectrophotometric assay for carboxyhaemoglobin in blood. Using the original procedure we have often observed a time-dependent change in the signal leading to unreliable results. By using phosphate-buffered-saline (pH 7.4) instead of ammonia to dissolve the deoxygenating agent (sodium dithionite), we obtained stable and reproducible second-derivative signals from which the percentage carboxyhaemoglobin in the patients' samples can be calculated.