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Role of the C-terminus in the biological activity of human interleukin 5

S Kodama1, N Tsuruoka, M Tsujimoto

  • 1Suntory Institute for Biomedical Research, Osaka, Japan.

Biochemical and Biophysical Research Communications
|July 31, 1991
PubMed

Alterations of the C-terminal amino acid sequence of recombinant human interleukin 5 (rhIL-5) caused significant changes in its biological activity. Removal of eight amino acids from the C-terminus of rhIL-5 by CNBr treatment led to a complete loss of biological activity as determined by the BCL1 cell IgM-inducing assay. Oxidation of Met residue located at C-terminus also resulted in a loss of activity. These results suggest that the C-terminal amino acids of rhIL-5 are crucial for its biological actions.

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