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Updated: Jul 4, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Single-beam homodyne SPIDER for multiphoton microscopy.

Jiha Sung¹, Bi-Chang Chen, Sang-Hyun Lim

¹Department of Chemistry and Biochemistry, University of Texas at Austin, 1 University Station A5300, Austin, Texas 78712, USA.

|July 3, 2008

Summary

A new spectral phase interferometry for direct electric field reconstruction (SPIDER) method uses a single phase-shaped laser beam for ultrafast laser pulse analysis. This technique enables precise spectral phase compensation in nonlinear microscopy applications.

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Last Updated: Jul 4, 2026

Conducting Multiple Imaging Modes with One Fluorescence Microscope

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Published on: October 28, 2018

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

Ultrafast optics
Nonlinear optics
Laser science

Background:

Accurate characterization of ultrafast laser pulses is crucial for nonlinear optical experiments.
Existing methods for spectral phase measurement can be complex or require multiple beams.

Purpose of the Study:

To develop a simplified and robust method for spectral phase measurement and compensation.
To enable precise control of laser pulse properties for advanced microscopy.

Main Methods:

A novel spectral phase interferometry for direct electric field reconstruction (SPIDER) approach is presented.
It utilizes a single phase-shaped laser beam, a phase pulse-shaping technique, and second-harmonic generation (SHG).
The method combines double-quadrature spectral interferometry with homodyne optical technique for SPIDER (HOT SPIDER).

Main Results:

The technique analytically retrieves the spectral phase of broadband laser pulses.
Arbitrary spectral phase at the sample position can be compensated with high precision (0.05 rad).
The method is demonstrated to be effective over the full width at half maximum (FWHM) of the laser spectrum.

Conclusions:

This new SPIDER version offers a simplified and efficient approach for ultrafast laser pulse characterization.
It is readily applicable to nonlinear microscopy, enhancing phase control capabilities.
The technique paves the way for improved resolution and performance in advanced optical imaging.