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Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

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Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples
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Published on: March 26, 2021

Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples.

Chia-Ming Lee1, Po-Yen Lin2, Yu-Ting Tseng1

  • 1Research Center for Applied Sciences, Academia Sinica, Taipei 11529, Taiwan.

Iscience
|June 22, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a versatile Bessel light sheet microscopy (BLX) system for imaging cleared tissues. The system enables high-resolution 3D visualization of developmental processes in mouse embryos and brains.

Keywords:
applied scienceschemistryimaging equipment

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Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

Area of Science:

  • Developmental Biology
  • Microscopy
  • Imaging Science

Background:

  • Advanced imaging techniques like tissue clearing and light-sheet microscopy are crucial for visualizing complex biological structures.
  • Understanding cell differentiation, tissue reorganization, and morphogenesis requires high-resolution 3D imaging methods.

Purpose of the Study:

  • To present a versatile Bessel light sheet microscopy (BLX) system optimized for whole-mount 3D imaging of cleared tissues.
  • To demonstrate the utility of the BLX system for visualizing microanatomical features in cleared mouse embryos and brains.

Main Methods:

  • Developed a Bessel light sheet microscopy (BLX) system compatible with immersion and capped air objectives.
  • Designed a protective lens cap to enable solvent-based clearing protocols with standard air objectives.
  • Utilized autofluorescence-based light-sheet imaging for cleared tissues.

Main Results:

  • Successfully visualized microanatomical features, including nephrons, in CUBIC-R-cleared mouse embryos.
  • Achieved cellular-resolution imaging of whole PEGASOS-cleared mouse brains using the capped BLX configuration.
  • Demonstrated the system's capability for autofluorescence-based imaging.

Conclusions:

  • The versatile BLX system facilitates high-resolution 3D imaging of cleared tissues.
  • The protective lens cap enhances compatibility with solvent-based clearing protocols.
  • The system's open-source nature and simplified design promote wider adoption in developmental biology research.