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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry
Published on: May 21, 2018
Trypsin purification by affinity binding to small unilamellar liposomes
J D Powers1, P K Kilpatrick, R G Carbonell
1Department of Chemical Engineering, North Carolina State University, Raleigh, North Carolina 27695-7905, USA.
Biotechnology and Bioengineering
|August 20, 1990
Summary
This study introduces a novel protein purification method using affinity-ligand-modified liposomes and ultrafiltration. The process efficiently purifies trypsin from complex mixtures with high yield and purity, demonstrating liposome stability and reusability.
Area of Science:
- Biochemistry
- Biotechnology
- Protein Chemistry
Background:
- Traditional protein purification methods can be inefficient and costly.
- Developing novel affinity-based purification techniques is crucial for isolating specific proteins.
Purpose of the Study:
- To describe and validate a novel protein purification process using affinity-ligand-modified liposomes and membrane ultrafiltration.
- To assess the efficiency, stability, and reusability of the affinity liposomes for trypsin purification.
Main Methods:
- Preparation of affinity liposomes by covalently attaching p-aminobenzamidine (PAB) to liposome surfaces.
- Utilizing membrane ultrafiltration for protein separation.
- Elution of bound trypsin using benzamidine.
Main Results:
- Achieved 68% recovery yield and 98% purity for trypsin purified from porcine pancreatic extract.
- Demonstrated stability of affinity-modified liposomes in complex mixtures.
- Confirmed retained trypsin binding capacity after multiple adsorption/elution cycles over 30 days.
Conclusions:
- The described liposome-based affinity purification process is effective for high-purity trypsin isolation.
- The affinity liposomes exhibit excellent stability and reusability, making the process economically viable.
- This novel method offers a promising alternative for protein purification in biotechnology and research.
