Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 4, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Continuous cell suspension processing using magnetically stabilized fluidized beds.

B E Terranova1, M A Burns

  • 1Genetics Institute, Andover, Massachusetts, 01003, USA.

Biotechnology and Bioengineering
|January 20, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding the school experiences of children and adolescents with serious chronic illness: a systematic meta-review.

Child: care, health and development·2017
Same author

Serological diagnosis of Leptospirosis in bovine serum samples using a microsphere immunoassay.

Veterinary record open·2016
Same author

The emergence of Leptospira borgpetersenii serovar Arborea as the dominant infecting serovar following the summer of natural disasters in Queensland, Australia 2011.

Tropical biomedicine·2014
Same author

Nasal cytokine responses to natural colds in asthmatic children.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology·2012
Same author

Serosurvey of leptospirosis and investigation of a possible novel serovar Arborea in farmed deer in New Zealand.

New Zealand veterinary journal·2011
Same author

Continuous affinity chromatography using a magnetically stabilized fluidized bed.

Biotechnology progress·2010
Same journal

Microbial Hydrolysates as Amino Acid Source in Cell Culture Media for Cellular Agriculture.

Biotechnology and bioengineering·2026
Same journal

LLM-Guided Parameter Optimization for Mechanistic CHO Cell Bioreactor Models.

Biotechnology and bioengineering·2026
Same journal

Three-Dimensional-Printed Polylactic Acid Scaffolds Coated With a Paeonol-Incorporated Gelatin/Bioactive Glass Composite Layer for Enhanced Osteogenic Performance.

Biotechnology and bioengineering·2026
Same journal

Recent Progress in Antimicrobial Peptides (AMPs) Towards Enhanced Selectivity and Reduced Cytotoxicity by Molecular Engineering.

Biotechnology and bioengineering·2026
Same journal

mZVI-Enhanced Mixed Nitrogen Removal in Klebsiella oxytoca via Coordinated Electron Transfer and Metabolic Reprogramming.

Biotechnology and bioengineering·2026
Same journal

Growth Model for Continuous Culture of a Hydrogen-Oxidizing Bacterium, Hydrogenophilus thermoluteolus Strain TH-1.

Biotechnology and bioengineering·2026
See all related articles

A magnetically stabilized fluidized bed (MSFB) effectively filters yeast cells from suspensions. This continuous countercurrent separator achieved up to 99% removal rates by controlling cell-support interactions.

Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Fluid Dynamics

Background:

  • Cell suspensions present challenges in continuous separation processes.
  • Magnetically stabilized fluidized beds (MSFB) offer unique fluid dynamics properties.
  • Developing efficient cell filtration methods is crucial for various biotechnological applications.

Purpose of the Study:

  • To investigate the behavior of cell suspensions in a continuous MSFB.
  • To design and evaluate a continuous countercurrent separator using MSFB technology.
  • To assess the filtration performance and identify key operational parameters.

Main Methods:

  • Experimental investigation of yeast cell suspension behavior in an MSFB.
  • Theoretical modeling to understand filtration mechanisms.

More Related Videos

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
09:03

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Published on: March 17, 2023

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

Related Experiment Videos

Last Updated: Jul 4, 2026

Automated Counterflow Centrifugal System for Small-Scale Cell Processing
04:49

Automated Counterflow Centrifugal System for Small-Scale Cell Processing

Published on: December 12, 2019

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow
09:03

Human Mesenchymal Stem Cell Processing for Clinical Applications Using a Closed Semi-Automated Workflow

Published on: March 17, 2023

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
10:23

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

Published on: December 13, 2016

  • Systematic variation of bed height, solids velocity, cell concentration, and liquid composition.

    • Main Results:

    • A continuous countercurrent separator was successfully constructed utilizing MSFB.
    • The MSFB demonstrated efficient filtration of yeast cells, achieving removal rates up to 99%.
    • Filtration performance was tunable by controlling cell/support interactions, with evidence of cell-cell binding and shadowing.

    Conclusions:

    • MSFB technology is a viable method for continuous cell separation.
    • The developed separator offers high efficiency and adjustable performance.
    • Mathematical modeling provides valuable insights into the filtration mechanisms within the MSFB.