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Updated: Jul 3, 2026

Scalable Isolation and Purification of Extracellular Vesicles from Escherichia coli and Other Bacteria
Published on: October 13, 2021
Flocculation of cell debris from ultrasonicated Escherichia coli
E Gellan1, G F Martin, R H Cumming
1Department of Chemical Engineering, North East Biotechnology Centre, Teesside Polytechnic, Middlesbrough, Cleveland TS1 3BA, United Kingdom.
Abstract:
Cell debris of Escherichia coli produced by ultrasonication was flocculated with acid. The debris settled at pH 3 and 4, but not at pH 2. Extensive protein precipitate formed in lysates at low pH. The results were compared with whole cells flocculated by acid.
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