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Related Concept Videos

Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

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Stable Aqueous Suspensions of Manganese Ferrite Clusters with Tunable Nanoscale Dimension and Composition
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Continuous protein separations in a magnetically stabilized fluidized bed using nonmagnetic supports.

A S Chetty1, M A Burns

  • 1E.I. DuPont Engineering Department, Newark, DE 19714-6090, USA.

Biotechnology and Bioengineering
|November 1, 1991
PubMed
Summary

Magnetically stabilized fluidized beds (MSFB) enable continuous protein separation using nonmagnetic affinity resins. This method offers efficient, countercurrent contact for recovering proteins like lysozyme.

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Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Biotechnology

Background:

  • Continuous separation processes are crucial for efficient protein purification.
  • Magnetically stabilized fluidized beds (MSFB) offer potential for enhanced mass transfer and continuous operation.
  • Stabilizing nonmagnetic materials in MSFB requires careful consideration of magnetic particle content and operating parameters.

Purpose of the Study:

  • To investigate the feasibility of using a magnetically stabilized fluidized bed (MSFB) for continuous protein separation.
  • To evaluate the performance of a nonmagnetic affinity resin within an MSFB system.
  • To determine the optimal conditions for stabilizing the MSFB with minimal magnetic particle content.

Main Methods:

  • Performed continuous protein separations using a magnetically stabilized fluidized bed (MSFB).
  • Employed a commercially available affinity adsorption resin lacking magnetically susceptible material.
  • Investigated the effect of magnetic particle concentration (as low as 20% by volume) on bed stabilization.
  • Analyzed parameters such as magnetic field strength and fluidization velocity.

Main Results:

  • Successfully stabilized nonmagnetic affinity resins in an MSFB at relatively low magnetic fields (<75 G).
  • Achieved continuous, countercurrent liquid-solid contact with mass-transfer efficiencies comparable to packed beds.
  • Demonstrated the ability to handle suspended cells or cell debris within the MSFB.
  • Successfully adsorbed and recovered lysozyme from a mixture with myoglobin using the affinity resin.

Conclusions:

  • Magnetically stabilized fluidized beds (MSFB) are effective for continuous protein separations using nonmagnetic affinity resins.
  • MSFB technology provides efficient mass transfer and continuous operation suitable for bioprocessing.
  • This approach allows for the purification of valuable proteins from complex biological mixtures.