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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
Published on: May 5, 2016
A fluorescence-based fiber optic biosensor for the flow-injection analysis of penicillin
X Xie1, A A Suleiman, G G Guilbault
1Department of Chemistry, University of New Orleans, New Orleans, LA 70148, USA.
Abstract:
A penicillin fiber optic sensor is described. The sensor is based on co-immobilization of a pH indicator, fluorescein isothiocyanate (FITC), and penicillinase on a preactivated biodyne B membrane attached to the end of a bifurcated optical fiber. The characteristics of the sensor are investigated in conjunction with a flow injection analysis system. The proposed sensor is reversible and responds to penicillin in the concentration range of 1 x 10(-4) to 5 x 10(-2) mol/L. The application of this sensor to penicillin analysis in some pharmaceutical samples is demonstrated.

